7550 (ISO International Standard INTERNATIONAL ORGANIZATION FOR STANDARDIZATION MEXYHAPOAHAH OPrAHW3AMR nO CTAHAAPTW3AWUORGANISATION INTE RNATIONALE DE NORMALISATION Laboratory glassware -- Disposable micropipettes Verrerie de laboratoire -- Micropipettes a usage unique First edition - 1985-05-01 UDC 542.3 Ref. No. ISO 7550-1985 (E) ISO 7550-1985 ( Descriptors : laboratory equipment, laboratory glassware, pipettes, specifications, dimensions, tests, marking, colour codes. Price based on 5 pages Copyright International Organization for Standardization No r without license from IHS Not for Resale Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- mental and non-governmental, in liaison with ISO, also take part in the work. Draft international Standards adopted by the technical committees are circulated to the member bodies for approval before their acceptance as International Standards by the ISO Council. They are approved in accordance with iSO procedures requiring at least 75 % approval by the member bodies voting. International Standard ISO 7550 was prepared by Technical Committee ISO/TC 48, Laboratory glassware and related apparatus. ? International Organization for Standardization, 1985 Printed in Switzerland Copyright Internatinal Organizatin for Standardization ed without license from IHS Not for Resale ISO 7550-1985 (E) INTERNATIONAL STANDARD Laboratory glassware - Disposable micropipettes Scope and field of application 4 Basis of adjustment This International Standard specifies requirements for dispos- 4.1 Unit of volume able glass micropipettes adjusted to contain, suitable for general laboratory purposes. The details specified are in confor- The unit of volume is the cubic millimetre (mm3), for which the mity with ISO 8417. name microlitre (μl) may be used. 2 References NOTE -- The term microlitre (μl) is commonly used as a special name for the cubic milimetre (mm3), in accordance with the International ISo 719, Glass - Hydrolytic resistance of glass grains at 98°C - System of Units (SI). Method of test and classification. 4.2 Reference temperature Iso 1769, Laboratory glassware -- Pipettes -- Colour coding. The reference temperature, i.e. the temperature at which the Iso 3534, Statistics --- Vocabulary and symbols. pipette is intended to contain its nominal volume (nominal ISo 8417, Laboratory volumetric instruments Disposable capacity), is 20 °C. volumetric articles - Principles of design and construction. 1) NOTE - If the pipette is required for use in a country which has 3 Definitions ISO 8417 for tropical use), this value shall be substituted for 20 oc. For the purpose of this International Standard, the foilowing definitions apply. Dimensions, types, series of capacities 3.1 disposable micropipette : A micropipette intended to be used once only and then discarded. 5.1 Dimensions NOTE -- Such pipettes will only be expected to provide their specified performance during the original operation. The dimensions shall be as shown in tables 1 and 2. 3.2 accuracy (of a micropipette) : The closeness of agree- 5.2 Types ment between the nominal volume and the mean volume, ob- tained by applying the test procedure specified in clause 9, It is This International Standard specifies two types of pipettes. quantified by the inaccuracy of the mean. Type I : Disposable glass micropipettes with graduation 3.3 repeatability (of a micropipette) : The closeness of line and colour code (see figure 1) which contain their agreement between the individual volumes obtained by apply- volume when filled to the graduation line from the end away ing the test procedure specified in clause 9. lt is quantified by from the colour code. the imprecisi