论文标题
散装细菌悬浮液中运行量统计的定量表征
Quantitative characterization of run-and-tumble statistics in bulk bacterial suspensions
论文作者
论文摘要
我们引入了一种数值方法，以从中间散射函数的实验测量值中提取跑和摔倒动力学的参数。我们表明，在拉普拉斯领域进行程序是不可行的，而是采用了更新过程直接实时工作。我们首先验证了使用基于代理的模拟产生的数据的方法。这使我们能够确定准确测量运动参数所需的长度和时间尺度，包括翻滚频率和游泳速度。我们通过分别考虑单细胞和人口水平的速度可变性来比较跑步动态的不同模型。最后，我们将方法应用于使用差异动态显微镜获得的野生型大肠杆菌的实验数据。
We introduce a numerical method to extract the parameters of run-and-tumble dynamics from experimental measurements of the intermediate scattering function. We show that proceeding in Laplace space is unpractical and employ instead renewal processes to work directly in real time. We first validate our approach against data produced using agent-based simulations. This allows us to identify the length and time scales required for an accurate measurement of the motility parameters, including tumbling frequency and swim speed. We compare different models for the run-and-tumble dynamics by accounting for speed variability at the single-cell and population level, respectively. Finally, we apply our approach to experimental data on wild-type Escherichia coli obtained using differential dynamic microscopy.
