论文标题
通过考虑照明期间样品漂移的固定偶极子在高精度上定位
Localization of fixed dipoles at high precision by accounting for sample drift during illumination
论文作者
论文摘要
单分子定位显微镜取决于样品中单染料发射器的位置的精确定量。可以从每个分子检测到的光子数量提高了这种精度。因此，建议增加记录过程的照明时间。特别是在低温温度下记录会大大降低光漂白，从而使照明时间大幅度增加到几秒钟。作为一个缺点，显微镜不稳定性很可能在如此长的照明期间引入抖动，从而恶化了本地化精度。在本文中，我们从理论上证明了基准标记珠的平行记录以及一种新型的拟合方法，该方法对完整的漂移轨迹进行了解释，可以在很大程度上消除每帧数百纳米的漂移幅度的漂移效应。
Single molecule localization microscopy relies on the precise quantification of the position of single dye emitters in a sample. This precision is improved by the number of photons that can be detected from each molecule. It is therefore recommendable to increase illumination times for the recording process. Particularly recording at cryogenic temperatures dramatically reduces photobleaching and thereby allows a massive increase in illumination times to several seconds. As a downside, microscope instabilities may well introduce jitter during such long illuminations, deteriorating the localization precision. In this paper, we theoretically demonstrate that a parallel recording of fiducial marker beads together with a novel fitting approach accounting for the full drift trajectory allows for largely eliminating drift effects for drift magnitudes of several hundred nanometers per frame.
