论文标题
线性微型合成扫描仪的在线校准
Online calibration of a linear micro tomosynthesis scanner
论文作者
论文摘要
在旨在成像以10微米分辨率的几厘米大小的组织学样品的线性上层扫描仪中,扫描阶段的机械不稳定性(+/- 10微米)超过了图像系统的分辨率,因此有必要确定每个扫描阶段的轨迹,以避免使用模糊和肉体在图像中进行搜索,从而将图像用于图像中的图像。我们通过将一层随机分散的微玻璃珠或钙颗粒连接到样品阶段的底部来提供在线校准的方法。标记层易于生产,并且在校准过程中有效。
In a linear tomosynthesis scanner designed for imaging histologic samples of several centimeter size at 10 micrometer resolution, the mechanical instability of the scanning stage (+/-10 micrometers) exceeded the resolution of the image system, making it necessary to determine the trajectory of the stage for each scan to avoid blurring and artifacts in the images that would arise from the errors in the geometric information used in 3D reconstruction. We present a method for online calibration by attaching a layer of randomly dispersed micro glass beads or calcium particles to the bottom of the sample stage. The marker layer was easy to produce and proven effective in the calibration procedure.