论文标题
SPIM-FLOW，一种用于Hydra流体动力学研究的集成光页和微流体平台
SPIM-Flow, an integrated light-sheet and microfluidics platform for hydrodynamic studies of Hydra
论文作者
论文摘要
选择性平面照明显微镜（SPIM）或轻板是强大的三维成像方法。然而，与微流体的访问和接口显微镜仍然具有挑战性。与微流体的复杂接口限制了SPIM在研究自由移动多细胞生物的流体动力学方面的效用。我们开发了Spim-Flow，这是一个廉价的轻型平台，可以轻松地与微流体集成。我们使用SPIM-FLOW研究了毫米大小的腔室（4毫米宽，1.5毫米）中自由移动的Hydra息肉的流体动力学。我们对多种动物进行的最初实验，以芯片为食（franciscana nauplius用作食物），以及基线行为（例如，触手摇摆，延伸和弯曲）在系统内表示动物的健康。 SPIM启用了较大腔室内自由移动的动物和示踪剂珠（用于流体可视化）的简易成像。接下来，使用腔室，我们研究了Hydra对流动的反应。结果表明，动物通过弯曲和摇动触手在流动方向上对确定的流动做出了反应。最后，我们使用了先前描述的视频分析软件（FlowTrace）来可视化由（例如，涡旋生成）以及周围（例如，由于流动）生成的路径线。这些结果证明了Spim-Flow研究自由移动动物的流体动力学的实用性。
Selective plane illumination microscopy (SPIM), or light sheet, is a powerful three-dimensional imaging approach. However, access to and interfacing microscopes with microfluidics have remained challenging. Complex interfacing with microfluidics has limited the SPIM's utility in studying the hydrodynamics of freely moving multicellular organisms. We developed SPIM-Flow, an inexpensive light sheet platform that enables easy integration with microfluidics. We used SPIM-Flow to study the hydrodynamics of freely moving Hydra polyps in millimeter-sized chambers (4 mm wide, 1.5 mm height). Our initial experiments across multiple animals, feeding on a chip (Artemia franciscana nauplius used as food), and baseline behaviors (eg., tentacle swaying, elongation, and bending) indicated animals' health inside the system. SPIM enabled easy imaging of the freely moving animal and tracer beads (for fluid visualizations) inside the larger chambers. Next, using the chambers, we investigated Hydra's response to flow. Results suggest that animals responded to established flow by bending and swaying their tentacles in the flow direction. Finally, we used a previously described video analysis software (FlowTrace) to visualize path lines generated by (e.g., vortex generated) and around (e.g., due to flow) Hydra. These results demonstrated the SPIM-Flow's utility to study the hydrodynamics of freely moving animals.
