论文标题
Mapache：绘制古代DNA的灵活管道
Mapache: a flexible pipeline to map ancient DNA
论文作者
论文摘要
摘要：将古代DNA映射到参考基因组是具有挑战性的，因为它涉及许多步骤，是耗时的，并且必须在研究中重复以评估提取物和图书馆的质量；结果，需要自动化映射以可重复的方式处理大量数据。我们提出了Mapache，这是一种灵活，健壮且可扩展的管道，以可重复的方式绘制，量化和估算古代和当今的DNA。 Mapache是在工作流管理器Snakemake中实现的，并经过优化用于低空消耗，可以有效地（RE）映射大型数据集，例如参考面板，多个提取物和库。
Summary: Mapping ancient DNA to a reference genome is challenging as it involves numerous steps, is time-consuming and has to be repeated within a study to assess the quality of extracts and libraries; as a result, the mapping needs to be automatized to handle large amounts of data in a reproducible way. We present mapache, a flexible, robust, and scalable pipeline to map, quantify and impute ancient and present-day DNA in a reproducible way. Mapache is implemented in the workflow manager Snakemake and is optimized for low-space consumption, allowing to efficiently (re)map large data sets such as reference panels and multiple extracts and libraries.
