论文标题
3D散射显微镜样品以评估层析成像阶段显微镜技术中的定量精度
3D scattering microphantom sample to assess quantitative accuracy in tomographic phase microscopy techniques
论文作者
论文摘要
在本文中，我们提出了一种鲁棒评估具有多个散射3D打印的微疗法的各种断层摄影显微镜（TPM）方法的定量准确性，具有已知的几何形状和折射率分布。我们通过制造多个散发幻影并比较3D折射率结果来证明这一方法，这些幻影结果是从三种TPM重建方法中输出的，具有可见的和近红外波长。这些方法之一假设样品是弱散射的，而其他两个则考虑了多个散射。这项研究可以很容易地扩展到更复杂的显微镜，该微体能够捕获现实世界散射物体（例如组织）的散射特征。
In this paper we present a method to robustly evaluate the quantitative accuracy of various tomographic phase microscopy (TPM) methods with a multiple scattering 3D-printed microphantom with known geometry and refractive index distribution. We demonstrate this method by fabricating a multiple-scattering phantom and comparing 3D refractive index results that are output from three TPM reconstruction methods operating with visible and near-infrared wavelengths. One of these methods assumes the sample to be weak-scattering, while the other two take multiple scattering into account. This study can be readily extended to more complex microphantoms fabricated to more closely capture scattering characteristics of real-world scattering objects, such as tissue.
