论文标题
猴蛋白质病毒的当前和透视传感方法：婴儿期的重新变体
Current and perspective sensing methods for monkeypox virus: a reemerging zoonosis in its infancy
论文作者
论文摘要
目的审查专门评估当前的Monkeypox病毒（MPXV）检测方法，讨论其优点和缺点，并为问题提供推荐的解决方案。 方法通过在PubMed，Web of Science，Google Scholar，ResearchGate和Science直接搜索英语发表的文章中，通过使用“ Monkeypox病毒”或“ Poxvirus”术语以及“诊断”来确定本综述的文献。 “ PCR”； “实时PCR”； “灯”; “ RPA”； “免疫测定”； “重新出现”； “ Biothreat”；也通过跟踪相关论文的引用，“流行”和“多国爆发”。评论中包括最相关的文章。 结果我们的文献综述表明，PCR是MPXV检测的黄金标准方法。此外，据报道，循环介导的等热扩增（LAMP）和重组酶聚合酶扩增（RPA）是PCR的替代方法。免疫诊断，整个颗粒检测和基于图像的检测是基于非核酸的MPXV检测方式。 结论PCR易于利用并适应快速响应爆发，但是基于PCR的MPXV检测方法可能不适合边缘化设置。在MPXV诊断方面的创新取得了有限的进展，为该病毒开发新的检测技术提供了空间。
Objectives The review is dedicated to evaluate the current monkeypox virus (MPXV) detection methods, discuss their pros and cons, and provide recommended solutions to the problems. Methods The literature for this review is identified through searches in PubMed, Web of Science, Google Scholar, ResearchGate, and Science Direct advanced search for articles published in English without any start date until June, 2022, by use of the terms "monkeypox virus" or "poxvirus" along with "diagnosis"; "PCR"; "real-time PCR"; "LAMP"; "RPA"; "immunoassay"; "reemergence"; "biothreat"; "endemic", and "multi-country outbreak" and also, by tracking citations of the relevant papers. The most relevant articles are included in the review. Results Our literature review shows that PCR is the gold standard method for MPXV detection. In addition, loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) have been reported as alternatives to PCR. Immunodiagnostics, whole particle detection, and image-based detection are the non-nucleic acid-based MPXV detection modalities. Conclusions PCR is easy to leverage and adapt for a quick response to an outbreak, but the PCR-based MPXV detection approaches may not be suitable for marginalized settings. Limited progress has been made towards innovations in MPXV diagnostics, providing room for the development of novel detection techniques for this virus.
