论文标题
λ/8分辨率的高保真远场显微镜
High-fidelity far-field microscopy at λ/8 resolution
论文作者
论文摘要
远场超分辨率显微镜的出现使纳米级成像的可能性恢复了活力。利用点扫描的远场超分辨率的方法通常取决于空间减少聚焦点的大小。但是，集中的斑点总是以极高的光效率来实现高分辨率，以探测大型内衣和高强度的旁观，这限制了纳米级成像中的应用，并可能导致样品的误解。在这里，我们报告了一个尖锐的探测点，衍射效率为3.76％，分辨率为38％的通风斑点尺寸，并在实验中由二维多级衍射光学元素（DOE）辅助。通过打破圆形0-π二进制结构升级透镜的限制，在同一分辨率下，至少两个数量级的衍射效率通过至少两个数量级提高了。为了消除高强度旁观的影响，根据先前的知识，基于修改的反卷积算法重建了高保真图像。最后，构建了高保真远场显微镜（HIFI-FM），实验结果表明，HIFI-FM可以在获得高保真度的同时分辨出比69 nm更好的空间复杂样品。
The emergence of far-field super-resolution microscopy has rejuvenated the possibility for nanoscale imaging. Approaches to far-field super-resolution that utilize point scanning often depends on spatially reducing the size of the focused spot. However, the focused spot always achieves high resolution at the expense of extremely low light efficiency for the probing mainlobe and high-intensity sidelobes, which limits the applications in nanoscale imaging and might cause misinterpretation of samples. Here we report a sharp probing spot with the diffraction efficiency of 3.76% at the resolution of 38% of the Airy spot size assisted by the two-dimensional multi-level diffractive optical element (DOE) experimentally. The diffraction efficiency of DOE is improved by at least two orders of magnitude at the same resolution by breaking the limitation of circular 0-π binary structure superoscillatory lens. To eliminate the influence of the high-intensity sidelobes, high-fidelity images are reconstructed based on the modified deconvolution algorithm by virtue of the prior-knowledge. Finally, high-fidelity far-field microscopy (HiFi-FM) is constructed and experimental results show that HiFi-FM allows the resolution of spatially complex samples better than 69 nm while acquiring high fidelity.
