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Data augmentation with \textbf{Mixup} has been proven an effective method to regularize the current deep neural networks. Mixup generates virtual samples and corresponding labels at once through linear interpolation. However, this one-stage generation paradigm and the use of linear interpolation have the following two defects: (1) The label of the generated sample is directly combined from the labels of the original sample pairs without reasonable judgment, which makes the labels likely to be ambiguous. (2) linear combination significantly limits the sampling space for generating samples. To tackle these problems, we propose a novel and effective augmentation method based on global clustering relationships named \textbf{Global Mixup}. Specifically, we transform the previous one-stage augmentation process into two-stage, decoupling the process of generating virtual samples from the labeling. And for the labels of the generated samples, relabeling is performed based on clustering by calculating the global relationships of the generated samples. In addition, we are no longer limited to linear relationships but generate more reliable virtual samples in a larger sampling space. Extensive experiments for \textbf{CNN}, \textbf{LSTM}, and \textbf{BERT} on five tasks show that Global Mixup significantly outperforms previous state-of-the-art baselines. Further experiments also demonstrate the advantage of Global Mixup in low-resource scenarios.