论文标题
在上转换纳米颗粒中利用动态非线性进行超分辨率成像
Exploiting dynamic nonlinearity in upconversion nanoparticles for super-resolution imaging
论文作者
论文摘要
单光束超分辨率显微镜,也称为超级线性显微镜,利用共聚焦显微镜中荧光探针的非线性反应。该技术不需要复杂的专用系统,光场调制或光束塑造。在这里,我们提出了一种通过调节图像采集过程中的激发强度来增强超线性显微镜空间分辨率的策略。该调制会在上转换纳米颗粒(UCNP)中诱导动态光学非线性,从而导致所获得图像中较高的空间频率信息的变化。可以通过提出的加权有限差成像算法从原始荧光图像中提取高阶信息,以生成比超线显微镜图像更高分辨率的图像。我们将这种方法应用于衍射限制区域内的两个相邻纳米颗粒,将分辨率提高到130 nm。这项工作提出了一个新的范围,用于在超分辨率纳米镜检查中开发动态非线性荧光探针。
Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or beam shaping. Here, we present a strategy to enhance spatial resolution of superlinear microscopy by modulating excitation intensity during image acquisition. This modulation induces dynamic optical nonlinearity in upconversion nanoparticles (UCNPs), resulting in variations of higher spatial-frequency information in the obtained images. The high-order information can be extracted with a proposed weighted finite difference imaging algorithm from raw fluorescence images, to generate an image with a higher resolution than superlinear microscopy images. We apply this approach to resolve two adjacent nanoparticles within a diffraction-limited area, improving the resolution to 130 nm. This work suggests a new scope for developing dynamic nonlinear fluorescent probes in super-resolution nanoscopy.