论文标题
使用单链DNA粘合到单层石墨烯场现场效应晶体管上的单链DNA，冠状病毒RNA传感器
Coronavirus RNA Sensor Using Single-Stranded DNA Bonded to Sub-Percolated Gold Films on Monolayer Graphene Field-Effect Transistors
论文作者
论文摘要
信使核糖核酸（mRNA）的电检测是一种有前途的方法，可以增强转录组学和疾病诊断，因为其敏感性，速度和模块化。这里报告的是一个快速的SARS-COV-2 mRNA生物传感器（<1分钟），检测限为1 am，动态范围为4个数量级，线性灵敏度为每摩尔十年22 mV。这些优异数字是在源自p-typeon上90 nm二氧化硅上的90 nm二氧化硅上的商业四英寸石墨烯中衍生出的商业四英寸石墨烯的光质图案单层石墨烯场晶体晶体管（FET）获得的。然后，为了促进mRNA杂交，将石墨烯传感台面与超薄的亚渗透阈值金膜覆盖，用于粘合3'-硫式的单链单链脱氧核糖核酸（SSDNA）探针，辅以与SARS-COV-2-Cov-2-2-2-2-2-2-2-2核蛋白酶磷酸蛋白（N）基因。亚渗透金用于最大程度地减少石墨烯材料与表面杂交事件之间的距离。石墨烯FET的液体转移特性反复显示狄拉克电压与多核苷酸的拷贝数之间的相关性。石墨烯FET上的超薄渗透金膜有助于二维电子气体（2DEG）mRNA生物传感器，用于转录组分析。
Electrical detection of messenger ribonucleic acid (mRNA) is a promising approach to enhancing transcriptomics and disease diagnostics because of its sensitivity, rapidity, and modularity. Reported here is a fast SARS-CoV-2 mRNA biosensor (<1 minute) with a limit of detection of 1 aM, and dynamic range of 4 orders of magnitude and a linear sensitivity of 22 mV per molar decade. These figures of merit were obtained on photoresistlessly patterned monolayer graphene field-effect transistors (FETs) derived from commercial four-inch graphene on 90 nm of silicon dioxide on p-type silicon. Then, to facilitate mRNA hybridization, graphene sensing mesa were coated with an ultrathin sub-percolation threshold gold film for bonding 3'-thiolated single-stranded deoxyribonucleic acid (ssDNA) probes complementary to SARS-CoV-2 nucleocapsid phosphoprotein (N) gene. Sub-percolated gold was used to minimize the distance between the graphene material and surface hybridization events. The liquid-transfer characteristics of the graphene FETs repeatedly shows correlation between the Dirac voltage and the copy number of polynucleotide. Ultrathin percolated gold films on graphene FETs facilitate two-dimensional electron gas (2DEG) mRNA biosensors for transcriptomic profiling.
