论文标题
与超分辨率显微镜和单粒子分析的分子复合物映射分子复合物
Mapping molecular complexes with Super-Resolution Microscopy and Single-Particle Analysis
论文作者
论文摘要
了解超分子络合物的结构可洞悉其功能能力以及如何在疾病中调节它们。超分辨率显微镜（SRM）通过以分子特异性在纳米级上解决超微结构细节来执行此任务。但是，诸如贴标签之类的技术限制排除了其提供完整结构的能力。单粒子分析（SPA）通过结合来自相同结构的多个图像并产生平均模型的信息来克服这一限制，从而有效增强了图像重建的分辨率和覆盖范围。这篇评论强调了使用SRM-SPA的重要研究，证明了它如何通过以前的细节阐明关键生物学结构的特征来扩展我们的知识。
Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM-SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail.
