论文标题
色聚焦层析成像
Chromatic Confocal Tomography
论文作者
论文摘要
共聚焦显微镜是世界各地细胞研究实验室的主干，但不幸的是，成像仅限于单个平面。色焦点显微镜提供了同时对多个平面进行成像的可能性，从而提供了成像速度的多种增加，而消除了对Z轴扫描的需求。标准的色素共聚焦系统的成像范围有限，数百微米的阶数限制了其应用。在这项工作中，使用单个硒化镜头，我们演示了一个色彩共聚焦断层扫描系统，该系统的成像范围为18 mm，平均侧面分辨率为2.46微米，另一个具有1.55 mm成像范围的系统，其平均横向分辨率为0.86微分。这样做，我们将色素共聚焦系统的成像范围从微尺到厘米制度提高，大约有两个数量级的改善。提出的方法可以是生物样品的共聚焦成像或工业样品表面分析的强大工具。
Confocal microscopy is the backbone of cellular research labs across the world but unfortunately, the imaging is restricted to a single plane. Chromatic confocal microscopy offers the possibility to image multiple planes simultaneously thus providing a manifold increase in the imaging speed whereas eliminating the need for z-axis scanning. Standard chromatic confocal systems have a limited imaging range of the order of a few hundreds of micrometers which limits their applications. In this work, using a single zinc selenide lens, we demonstrate a chromatic confocal tomography system that has an imaging range of 18 mm with an average lateral resolution of 2.46 microns and another system with a 1.55 mm imaging range with 0.86 microns average lateral resolution. Doing so, we have pushed the imaging range of chromatic confocal systems from micrometer to centimeter regime, an improvement of approximately two orders of magnitude. The proposed approach can be a powerful tool for confocal imaging of biological samples or surface profiling of industrial samples.
