论文标题
测量与单丝跟踪的交联和纠缠半串联聚合物网络的结构参数
Measuring structural parameters of crosslinked and entangled semiflexible polymer networks with single-filament tracing
论文作者
论文摘要
单丝跟踪一直是直接确定纠缠聚合物网络的几何和机械性能的宝贵工具。但是，系统地验证示踪剂细丝的刚度或其与周围网络的分子相互作用如何影响这些参数的测量，这对于已建立的实验系统是不可能的。在这里，我们使用机械可编程的DNA纳米管，这些纳米管嵌入了交联和纠缠的F-肌动蛋白网络以及合成DNA网络中，以测量相对于示踪剂的刚度，以测量管子宽度和网状尺寸（如管宽度和网状尺寸）。虽然我们仅基于空间相互作用来确认从模型得出的一些预测，但我们的结果表明应扩展这些模型以说明其他丝间相互作用，从而描述了实际聚合物网络的行为。
Single-filament tracing has been a valuable tool to directly determine geometrical and mechanical properties of entangled polymer networks. However, systematically verifying how the stiffness of the tracer filament or its molecular interactions with the surrounding network impacts the measurement of these parameters has not been possible with the established experimental systems. Here, we use mechanically programmable DNA nanotubes embedded in crosslinked and entangled F-actin networks, as well as in synthetic DNA networks, in order to measure fundamental, structural network properties like tube width and mesh size with respect to the stiffness of the tracers. While we confirm some predictions derived from models based purely on steric interactions, our results indicate that these models should be expanded to account for additional inter-filament interactions, thus describing the behavior of real polymer networks.
