论文标题
串行电子衍射数据处理用衍射和水晶处理
Serial Electron Diffraction Data Processing with diffractem and CrystFEL
论文作者
论文摘要
串行电子衍射（Serialed）是一种新兴技术，该技术将串行X射线晶体学的快照数据收集模式应用于三维电子衍射（3D ED），以进行常规旋转方法。与串行X射线晶体学类似相似，这种方法即使对于最敏感的样品也几乎完全没有辐射损伤影响，并且可以高水平自动化。然而，序列化还需要新的数据处理技术，这些技术结合了现有的旋转电子衍射和串行X射线晶体学的管道以及一些更特殊的解决方案，以针对串行中引起的挑战。在这里，我们介绍了用于串行数据的分析管道，并使用Crystfel和Diffractem程序软件包实现了其实现。详细的示例以广泛的补充代码提供。
Serial electron diffraction (SerialED) is an emerging technique, which applies the snapshot data-collection mode of serial X-ray crystallography to three-dimensional electron diffraction (3D ED), forgoing the conventional rotation method. Similarly to serial X-ray crystallography, this approach leads to almost complete absence of radiation damage effects even for the most sensitive samples, and allows for a high level of automation. However, SerialED also necessitates new techniques of data processing, which combine existing pipelines for rotation electron diffraction and serial X-ray crystallography with some more particular solutions for challenges arising in SerialED specifically. Here, we introduce our analysis pipeline for SerialED data, and its implementation using the CrystFEL and diffractem program packages. Detailed examples are provided in extensive supplementary code.
