论文标题
连接热力学和蛋白质稳定性的测量
Linking thermodynamics and measurements of protein stability
论文作者
论文摘要
我们回顾了分析平衡蛋白展开实验的背景，理论和一般方程，重点是变性和热诱导的展开。主要重点是可逆折叠/展开过渡的热力学以及可用于提取热力学参数的实验方法。我们强调了建模折叠平衡如何取决于扰动变量（例如温度或变性浓度）的重要性，以及在实验性观察物中对基准进行建模的重要性。
We review the background, theory and general equations for the analysis of equilibrium protein unfolding experiments, focusing on denaturant and heat-induced unfolding. The primary focus is on the thermodynamics of reversible folding/unfolding transitions and the experimental methods that are available for extracting thermodynamic parameters. We highlight the importance of modelling both how the folding equilibrium depends on a perturbing variable such as temperature or denaturant concentration, and the importance of modelling the baselines in the experimental observables.
