论文标题
一种评估结构化照明显微镜（SIM）的时空分辨率的方法
A method for assessing the spatiotemporal resolution of Structured Illumination Microscopy (SIM)
论文作者
论文摘要
提出了一种方法来评估结构化照明显微镜（SIM）的时间分辨率，通过跟踪不同空间频率成分的幅度，并将它们与时间振荡的基地真相进行比较。该方法用于洞悉SIM的性能限制，以及替代性重建技术（称为“滚动SIM”），声称可以改善时间分辨率。结果表明，SIM的时间分辨率在低空间频率和高空间频率之间差异很大，尽管在几个备受瞩目的论文和商业显微镜软件中使用了，但滚动SIM卡与常规SIM相比，时间分辨率没有增加。
A method is proposed for assessing the temporal resolution of Structured Illumination Microscopy (SIM), by tracking the amplitude of different spatial frequency components over time, and comparing them to a temporally-oscillating ground-truth. This method is used to gain insight into the performance limits of SIM, along with alternative reconstruction techniques (termed 'rolling SIM') that claim to improve temporal resolution. Results show that the temporal resolution of SIM varies considerably between low and high spatial frequencies, and that, despite being used in several high profile papers and commercial microscope software, rolling SIM provides no increase in temporal resolution over conventional SIM.
