论文标题
牙槽模拟物周期性应变及其对细胞层形成的影响
Alveolar mimics with periodic strain and its effect on the cell layer formation
论文作者
论文摘要
我们报告了在芯片上开发肺泡空气组织接口的新模型。该模型由一系列由微型框架支撑的明胶纳米纤维的悬浮式六边形单层和用于斑块积分的微流体设备。通过在200-1000 pa的范围内，相对于500 UM的单层直径，悬浮的单层在空气界面上的中心位移为40-80 UM的中心位移为40-80 UM，该位移对应于与2-10％的线性应变相吻合的生理应变范围，该位移与Lung Alveoli的物理学应变范围相吻合。单层孵育时间1-3天在单层上的A549细胞的培养物在模型中显示出生存力。我们在1小时内以0.2 Hz的频率在0.2 Hz的频率下施加了5％的周期性应变。我们发现细胞强烈耦合到纳米纤维，但是菌株减少了肌动蛋白细胞骨架的耦合和诱导的重塑，从而导致更好的组织形成。我们的模型可以用作肺部研究的多功能工具，例如吸入毒理学和治疗。
We report on the development of a new model of alveolar air-tissue interface on a chip. The model consists of an array of suspended hexagonal monolayers of gelatin nanofibers supported by microframes and a microfluidic device for the patch integration. The suspended monolayers are deformed to a central displacement of 40-80 um at the air-liquid interface by application of air pressure in the range of 200-1000 Pa. With respect to the diameter of the monolayers that is 500 um, this displacement corresponds to a linear strain of 2-10% in agreement with the physiological strain range in the lung alveoli. The culture of A549 cells on the monolayers for an incubation time 1-3 days showed viability in the model. We exerted a periodic strain of 5% at a frequency of 0.2 Hz during 1 hour to the cells. We found that the cells were strongly coupled to the nanofibers, but the strain reduced the coupling and induced remodeling of the actin cytoskeleton, which led to a better tissue formation. Our model can serve as a versatile tool in lung investigations such as in inhalation toxicology and therapy.
