论文标题
频率加倍的飞秒ER掺杂的纤维激光器，用于两光子激发荧光成像
Frequency-doubled femtosecond Er-doped fiber laser for two-photon excited fluorescence imaging
论文作者
论文摘要
具有可调脉冲重复速率的飞秒频率加倍的ERBIUM掺杂的纤维激光器，并将其应用于两光子激发荧光显微镜中。全纤维激光系统在1560 nm波长下提供基本脉冲，第二次谐波生成22 fs持续时间，导致1.35 NJ，60 fs脉冲在780 nm处。重复率是通过放大器链中内置的脉冲拾取器单元来调整的，直接为1至12 MHz之间的任何选择的重复速率提供了转换限制脉冲。我们采用激光源来驱动扫描的两光激发荧光显微镜，以驱动体内大鼠皮肤和其他样品以各种脉冲重复速率进行成像。由于紧凑，易于操作和合适的脉冲特性，激光源可以被视为在生物医学成像中的Ti：蓝宝石激光器的有吸引力的替代方法。
A femtosecond frequency-doubled Erbium-doped fiber laser with adjustable pulse repetition rate is developed and applied in two-photon excited fluorescence microscopy. The all-fiber laser system provides the fundamental pulse at 1560 nm wavelength with 22 fs duration for the second harmonic generation, resulting in 1.35 nJ, 60 fs pulses at 780 nm. The repetition rate is adjusted by a pulse picker unit built-in within the amplifier chain, directly providing transform-limited pulses for any chosen repetition rate between 1 and 12 MHz. We employed the laser source to drive a scanning two-photon excited fluorescence microscope for ex vivo rat skin and other samples imaging at various pulse repetition rates. Due to compactness, ease of operation, and suitable pulse characteristics the laser source can be considered as an attractive alternative for Ti:sapphire laser in biomedical imaging.
