论文标题
共聚焦显微镜中极端交叉极化灭绝的物理起源
The physical origins of extreme cross-polarization extinction in confocal microscopy
论文作者
论文摘要
共聚焦显微镜是固态物理学和纳米光子学中生物系统的必不可少的成像工具。使用共聚焦显微镜可以进行谐振荧光实验,其中发射光具有与激发激光相同的波长。在线性交叉极化条件下进行了具有挑战性的实验,拒绝了检测器的激光光。在这项工作中,我们发现了尚未解释的高极化排斥比的起源的物理机制,这使这些测量成为可能。我们在实验和理论中都表明,将反射表面(即放置在偏光剂和分析仪之间的横梁切割和镜子)与共聚焦排列结合使用,这解释了巨大的交叉极化灭绝率为10^8及以后。我们绘制了偏振光高斯光束的模态转换。我们在交叉极化条件下发现反射梁的强度“孔”。我们将其解释为Imbert-Fedorov效应的表现,它根据其极化的螺旋性偏离了光束。这意味着,由于我们的交叉极化黑场方法,此处从通常观察到的纳米尺寸到千分尺尺度上扩增了这种拓扑作用。我们确认了这些实验发现,这些发现是多种市售的镜子和偏振组件,从而在许多实验中实现了实际实现。
Confocal microscopy is an essential imaging tool for biological systems, in solid-state physics and nano-photonics. Using confocal microscopes allows performing resonant fluorescence experiments, where the emitted light has the same wavelength as the excitation laser. Theses challenging experiments are carried out under linear cross-polarization conditions, rejecting laser light from the detector. In this work we uncover the physical mechanisms that are at the origin of the yet unexplained high polarization rejection ratio which makes these measurements possible. We show in both experiment and theory that the use of a reflecting surface (i.e. the beam-splitter and mirrors) placed between the polarizer and analyzer in combination with a confocal arrangement explains the giant cross-polarization extinction ratio of 10^8 and beyond. We map the modal transformation of the polarized optical Gaussian beam. We find an intensity 'hole' in the reflected beam under cross-polarization conditions. We interpret this as a manifestation of the Imbert-Fedorov effect, which deviates the beam depending on its polarization helicity. This implies that this topological effect is amplified here from the usually observed nanometer to the micrometer scale due to our cross-polarization dark field methods. We confirm these experimental findings for a large variety of commercially available mirrors and polarization components, allowing their practical implementation in many experiments.