论文标题
朝着定义细胞外囊泡大小，浓度，折射率和表位丰度的参考材料
Towards defining reference materials for extracellular vesicle size, concentration, refractive index and epitope abundance
论文作者
论文摘要
细胞外囊泡（EV）的准确表征对于探索其诊断和治疗应用至关重要。随着电动汽车研究领域的发展，也用来表征它们的技术也是如此。这些技术的标准化需要参考材料的开发。这项工作是从英国伯明翰的ISEV 2017生物标志物研讨会发起的，并在比利时根特的ISEV 2019标准化研讨会上进行了进一步的讨论，旨在阐明需要哪些参考材料，并且目前可以使用哪些参考材料来标准化常用的分析平台，以表征EV，浓度，浓度，折射式指数，折射率和ePepepepepece和Epepepepepers和Epepepepepepers and Epepepepepers和Epepepepepepers和Epepepepepepers。由于其主要使用，因此将特定的重点放在光学方法纳米颗粒跟踪分析和流式细胞仪上。
Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference materials is required for the standardization of these techniques. This work, initiated from the ISEV 2017 Biomarker Workshop in Birmingham, UK, and with further discussion during the ISEV 2019 Standardization Workshop in Ghent, Belgium, sets out to elucidate which reference materials are required and which are currently available to standardize commonly used analysis platforms for characterizing EV size, concentration, refractive index, and epitope expression. Due to their predominant use, a particular focus is placed on the optical methods nanoparticle tracking analysis and flow cytometry.
