论文标题
使用光学镊子检测局部细胞膜斜率的次级波动
Detection of sub-degree fluctuations of the local cellmembrane slope using optical tweezers
论文作者
论文摘要
已经使用高分辨率显微镜和聚焦光广泛研究了细胞膜的正常热波动，尤其是在细胞的外围区域。我们使用非特异性连接到细胞膜的单个探针粒子，以确定功率谱密度与（频率）^（ - 1.66）成正比，在5 Hz至1 kHz的范围内。我们还使用一种新技术来同时确定膜的斜率波动，以确定被困在线性极化光学镊子中的双重探针粒子的俯仰运动。在此过程中，我们还开发了使用光学镊子鉴定高分辨率旋转到高分辨率的技术。我们发现，斜率波动的功率谱与1/（频率）成正比，我们在理论上也解释了这一点。我们发现我们可以直接从功率谱的系数中提取参数，尤其是在高频上，而不是用其他参数进行处理，从而提高估计的准确性。我们预测，确定螺距角度在球形粒子上的确定技术，以作为许多有趣的研究，以作为许多有趣的研究，以作为许多有趣的研究，以作为许多有趣的研究，
Normal thermal fluctuations of the cell membrane have been studied extensively using high resolution microscopy and focused light, particularly at the peripheral regions of a cell. We use a single probe particle attached non-specifically to the cell-membrane to determine that the power spectral density is proportional to (frequency)^(-1.66) in the range of 5 Hz to 1 kHz. We also use a new technique to simultaneously ascertain the slope fluctuations of the membrane by relying upon the determination of pitch motion of the birefringent probe particle trapped in linearly polarized optical tweezers. In the process, we also develop the technique to identify pitch rotation to a high resolu-tion using optical tweezers. We find that the power spectrum of slope fluctuations is proportional to 1/(frequency), which we also explain theoretically. We find that we can extract parameters like bending rigidity directly from the coefficient of the power spectrum particularly at high frequencies,instead of being convoluted with other parameters, thereby improving the accuracy of estimation.We anticipate this technique for determination of the pitch angle in spherical particles to high resolution as a starting point for many interesting studies using the optical tweezers
