论文标题
活细胞中的异常细胞的隔离：通过协作挑战弥合实验和现实模型之间的差距
Anomalous subdiffusion in living cells: bridging the gap between experiments and realistic models through collaborative challenges
论文作者
论文摘要
细胞的寿命受高度动力学的显微镜过程的控制。两个值得注意的例子是膜受体的扩散和控制基因表达速率的转录因子的动力学。已经出现了不同的荧光成像技术来研究分子动力学。其中，事实证明，荧光相关光谱（FCS）和单粒子跟踪（SPT）对我们对细胞动力学和功能的理解有用。 SPT和FCS的分析是一项持续的努力，尽管工作了数十年，但仍有许多进步要取得的进展。在本文中，我们快速概述用于分析细胞中异常扩散的现有技术，并提出协作挑战以促进最新的分析算法的发展。我们建议在开放且公平的挑战中向全世界的竞争对手提供标记（培训）和未标记（评估）模拟数据。目的是基于生物学上的指标提供统一的数据基准，以比较社区可用的扩散分析软件。
The life of a cell is governed by highly dynamical microscopic processes. Two notable examples are the diffusion of membrane receptors and the kinetics of transcription factors governing the rates of gene expression. Different fluorescence imaging techniques have emerged to study molecular dynamics. Among them, fluorescence correlation spectroscopy (FCS) and single-particle tracking (SPT) have proven to be instrumental to our understanding of cell dynamics and function. The analysis of SPT and FCS is an ongoing effort, and despite decades of work, much progress remains to be done. In this paper, we give a quick overview of the existing techniques used to analyze anomalous diffusion in cells and propose a collaborative challenge to foster the development of state-of-the-art analysis algorithms. We propose to provide labelled (training) and unlabelled (evaluation) simulated data to competitors all over the world in an open and fair challenge. The goal is to offer unified data benchmarks based on biologically-relevant metrics in order to compare the diffusion analysis software available for the community.
