论文标题
单发3D宽场荧光成像，带有计算微型介质
Single-Shot 3D Widefield Fluorescence Imaging with a Computational Miniature Mesoscope
论文作者
论文摘要
荧光成像对于生物学和神经科学是必不可少的。在自由表现的动物中对大规模成像的需求进一步驱动了微型显微镜（Miniscopes）的发展。但是，传统的显微镜 /米斯科植物固有地受到其有限的空间带宽产品的限制，浅水深度和无法解决3D分布式发射器的能力。在这里，我们提出了一个计算微型介质（CM $^2 $），它克服了这些瓶颈，并在8 $ \ times $ 7毫米$ $^2 $视野和25毫米的视野和2.25毫米视野中启用了单光3D成像，达到了7- $ $ $ M $ M的后期分辨率，并更好地分辨率$ M $ m $ m $ m $ m $ mμmmμmmμmmμmmμmmμmmμmmμmmμmmμmmμmmμmmμmsustolution。值得注意的是，CM $^2 $具有紧凑的轻巧设计，该设计集成了用于成像的Microlens阵列，并在一个平台中集成了激发阵列。通过通过算法增强光学元件的计算成像来启用其扩展的成像功能。我们通过实验验证了体积分布的荧光珠和纤维上的介观3D成像能力。我们进一步量化了体积散射和背景荧光对幻影实验的影响。
Fluorescence imaging is indispensable to biology and neuroscience. The need for large-scale imaging in freely behaving animals has further driven the development in miniaturized microscopes (miniscopes). However, conventional microscopes / miniscopes are inherently constrained by their limited space-bandwidth-product, shallow depth-of-field, and the inability to resolve 3D distributed emitters. Here, we present a Computational Miniature Mesoscope (CM$^2$) that overcomes these bottlenecks and enables single-shot 3D imaging across an 8 $\times$ 7-mm$^2$ field-of-view and 2.5-mm depth-of-field, achieving 7-$μ$m lateral resolution and better than 200-$μ$m axial resolution. Notably, the CM$^2$ has a compact lightweight design that integrates a microlens array for imaging and an LED array for excitation in a single platform. Its expanded imaging capability is enabled by computational imaging that augments the optics by algorithms. We experimentally validate the mesoscopic 3D imaging capability on volumetrically distributed fluorescent beads and fibers. We further quantify the effects of bulk scattering and background fluorescence on phantom experiments.
