论文标题
基因分型冠状病毒SARS-COV-2：方法和含义
Genotyping coronavirus SARS-CoV-2: methods and implications
论文作者
论文摘要
自2019年12月下旬在中国确定的，新兴的全球感染性冠状病毒19冠状病毒疾病是通过新型严重的急性呼吸综合症2（SARS-COV-2）对全球公共卫生和经济产生的关键威胁。该病毒经历了各种进化途径。为了理解SARS-COV-2的演变和传播，病毒分离株的基因分型非常重要。我们提出了一种使用完整基因组有效基因分型SARS-COV-2病毒的准确方法。该方法采用基因组分离株与SARS-COV-2参考基因组的多个序列比对。然后，通过Jaccard距离测量SNP基因型以跟踪病毒分离株的关系。来自世界的SARS-COV-2分离株的基因分型分析表明，特定的多重突变是当前流行期间主导的突变类型。我们的方法是一种有前途的工具，用于监测和跟踪其逐渐和局部遗传变异中致病病毒的流行。基因分型分析表明，编码S蛋白和RNA聚合酶，RNA原始酶和核蛋白的基因经历了频繁的突变。这些突变对于疾病控制中的疫苗开发至关重要。
The emerging global infectious COVID-19 coronavirus disease by novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) presents critical threats to global public health and the economy since it was identified in late December 2019 in China. The virus has gone through various pathways of evolution. For understanding the evolution and transmission of SARS-CoV-2, genotyping of virus isolates is of great importance. We present an accurate method for effectively genotyping SARS-CoV-2 viruses using complete genomes. The method employs the multiple sequence alignments of the genome isolates with the SARS-CoV-2 reference genome. The SNP genotypes are then measured by Jaccard distances to track the relationship of virus isolates. The genotyping analysis of SARS-CoV-2 isolates from the globe reveals that specific multiple mutations are the predominated mutation type during the current epidemic. Our method serves a promising tool for monitoring and tracking the epidemic of pathogenic viruses in their gradual and local genetic variations. The genotyping analysis shows that the genes encoding the S proteins and RNA polymerase, RNA primase, and nucleoprotein, undergo frequent mutations. These mutations are critical for vaccine development in disease control.
