论文标题
具有基于荧光的传输矩阵的散射介质中的非侵入性聚焦和成像
Non-invasive focusing and imaging in scattering media with a fluorescence-based transmission matrix
论文作者
论文摘要
在生物学显微镜中，光散射代表了深度图像的主要局限性。最近，已经开发了一套波前塑形技术，以操纵强烈无序的材料中的相干光。传输矩阵方法表明其能力有能力反向散射和有效聚焦光的效果。在实践中，通常使用侵入性检测器或低分辨率的声导星来测量矩阵。在这里，我们基于线性荧光引入了一种非侵入性和全光学策略，以重建放置在散射介质内的荧光对象。它包括使用低级别因素化和相检索算法将对象发出的不连贯模式结合。我们通过稳健和选择性的聚焦在实验中证明了该方法的效率。另外，从相同的测量值中，可以利用内存效应相关性与图像和重建扩展对象。这种方法为具有线性或非线性对比机制的散射介质打开了新的成像途径。
In biological microscopy, light scattering represents the main limitation to image at depth. Recently, a set of wavefront shaping techniques has been developed in order to manipulate coherent light in strongly disordered materials. The Transmission Matrix approach has shown its capability to inverse the effect of scattering and efficiently focus light. In practice, the matrix is usually measured using an invasive detector or low-resolution acoustic guide stars. Here, we introduce a non-invasive and all-optical strategy based on linear fluorescence to reconstruct the transmission matrices, to and from a fluorescent object placed inside a scattering medium. It consists in demixing the incoherent patterns emitted by the object using low-rank factorizations and phase retrieval algorithms. We experimentally demonstrate the efficiency of this method through robust and selective focusing. Additionally, from the same measurements, it is possible to exploit memory effect correlations to image and reconstruct extended objects. This approach opens up a new route towards imaging in scattering media with linear or non-linear contrast mechanisms.
