论文标题
连贯的反stokes拉曼通过单波形塑造在厚的生物组织中散射
Coherent anti-Stokes Raman scattering through thick biological tissues by single wavefront shaping
论文作者
论文摘要
连贯的抗Stokes拉曼散射（CARS）为非线性生物形象提供了许多优势，这要归功于其亚细胞空间分辨率和独特的化学特异性。它的工作原理需要两个入射的脉冲激光束，其频率不同，要集中在时空上，它们在生物组织的大深度繁殖时将质量却迅速恶化。目前尚不清楚汽车的深度限制和波前校正能力克服这些极限的能力。在这项工作中，我们利用了脉冲状态中散射介质的透射矩阵的光谱相关性能，以恢复两个远距离的事件波长最初不相关的远处事件车波长。使用单个空间光调节器的波前形状，我们通过厚实的小鼠脊髓组织恢复汽车的产生，最初由于散射而无法测量信号，并在数十微米的大量视野上进行了扫描点。
Coherent Anti Stokes Raman Scattering (CARS) offers many advantages for nonlinear bio-imaging, thanks to its sub-cellular spatial resolution and unique chemical specificity. Its working principle requires two incident pulsed laser beams with distinct frequencies to be focused in space and time, which focus quality however rapidly deteriorates when propagating at large depths in biological tissues. The depth limits of CARS and the capability of wavefront correction to overcome these limits are currently unknown. In this work we exploit the spectral correlation properties of the transmission matrix of a scattering medium in a pulsed regime, to recover coherent focusing for two distant incident CARS wavelengths which propagation is initially uncorrelated. Using wavefront shaping with a single spatial light modulator, we recover CARS generation through thick mice spinal cord tissues where initially no signal is measurable due to scattering, and demonstrate point scanning over large field of views of tens of micrometers.
