论文标题
稳定和非稳态条件下mRNA翻译中核糖体的动力学
Dynamics of ribosomes in mRNA translation under steady and non-steady state conditions
论文作者
论文摘要
DNA测序和荧光成像的最新进展使监测积极参与Messenger RNA（mRNA）翻译的核糖体动力学。在这里，我们使用逼真的动力学参数对这些实验进行建模。特别是我们介绍了分析表达式以描述以下三种情况：（a）新转录的mRNA的翻译，（b）稳定状态的翻译，特别是个体（标记）核糖体的动力学，以及（c）抑制翻译起始后的径流翻译。在（b）和（c）的情况下，我们开发了一个有效的培养基近似值，以在有效培养基中的单个标记核糖体来描述多核体动力学。这些预测与随机模拟非常吻合。
Recent advances in DNA sequencing and fluorescence imaging have made it possible to monitor the dynamics of ribosomes actively engaged in messenger RNA (mRNA) translation. Here, we model these experiments within the inhomogeneous totally asymmetric simple exclusion process (TASEP) using realistic kinetic parameters. In particular we present analytic expressions to describe the following three cases: (a) translation of a newly transcribed mRNA, (b) translation in the steady state and, specifically the dynamics of individual (tagged) ribosomes and (c) run-off translation after inhibition of translation initiation. In the cases (b) and (c) we develop an effective medium approximation to describe many-ribosome dynamics in terms of a single tagged ribosome in an effective medium. The predictions are in good agreement with stochastic simulations.
