论文标题
旋转磁盘 - 远程聚焦显微镜
Spinning Disk -- Remote Focusing Microscopy
论文作者
论文摘要
通过将远程聚焦与差分旋转磁盘光学切片相结合以快速获取细胞分辨率的实时样品图像来实现快速共聚焦成像。轴向和横向全宽度最大值分别小于5 um和490 nm，在130 um轴向范围内显示出256 x 128 UM视场。使用水指数校准载玻片来实现对齐方式，以最大程度地减少图像量失真。通过以1体积/s的形式获取24 um轴向范围内的图像体积来证明生物样品的应用，从而可以检测到铂雷尼氏菌幼虫中的基于钙的神经元活性。
Fast confocal imaging was achieved by combining remote focusing with differential spinning disk optical sectioning to rapidly acquire images of live samples at cellular resolution. Axial and lateral full width half maxima less than 5 um and 490 nm respectively are demonstrated over 130 um axial range with a 256 x 128 um field of view. A water-index calibration slide was used to achieve an alignment that minimises image volume distortion. Application to live biological samples was demonstrated by acquiring image volumes over a 24 um axial range at 1 volume/s, allowing for the detection of calcium-based neuronal activity in Platynereis dumerilii larvae.
