论文标题
基于模型的波前显微镜
Model-based wavefront shaping microscopy
论文作者
论文摘要
在现代显微镜中，越来越多地使用了波前塑形，以获得无均匀介质深处的无变形的高分辨率图像。波前塑形方法通常依赖于“ GuideStar”的存在，以找到最佳的波前以减轻光的散射。但是，在大多数生物医学应用中无法满足这种情况。在这里，我们介绍了一种新颖的，无指南的波前塑形方法，其中使用样本的数字模型计算最佳波前。用作计算输入的样品的折射率模型是由显微镜本身构造的。在原理成像实验的证明中，我们通过漫射介质证明了两光子荧光信号的大幅改进，表现优于最新的波前塑形技术，这是21倍。
Wavefront shaping is increasingly being used in modern microscopy to obtain distortion-free, high-resolution images deep inside inhomogeneous media. Wavefront shaping methods typically rely on the presence of a 'guidestar' in order to find the optimal wavefront to mitigate the scattering of light. However, this condition cannot be satisfied in most biomedical applications. Here, we introduce a novel, guidestar-free wavefront shaping method in which the optimal wavefront is computed using a digital model of the sample. The refractive index model of the sample, that serves as the input for the computation, is constructed in-situ by the microscope itself. In a proof of principle imaging experiment, we demonstrate a large improvement in the two-photon fluorescence signal through a diffuse medium, outperforming the state-of-the-art wavefront shaping techniques by a factor of 21.
