ICS 65.020.20 CCS B 05 45 广 西 壮 族 自 治 区 地方 标准 DB45/T 2754—2023 芒果品种鉴定技术规程 SSR分子标记法 Code of practice identification of mango varieties by S SR molecular marker method 2023 - 08 - 10发布 2023 - 09 - 30实施 广西壮族自治区市场监督管理局 发布 DB45/T 2754 —2023 I 目次 前言 ................................ ................................ ................. II 1 范围 ................................ ................................ ............... 1 2 规范性引用文件 ................................ ................................ ..... 1 3 术语和定义 ................................ ................................ ......... 1 4 缩略语 ................................ ................................ ............. 1 5 原理 ................................ ................................ ............... 2 6 材料和试剂 ................................ ................................ ......... 2 一般规定 ................................ ................................ ....... 2 6.1 试剂 ................................ ................................ ........... 2 6.2 溶液配制 ................................ ................................ ....... 2 6.3 7 仪器和设备 ................................ ................................ ......... 3 8 SSR引物序列信息 ................................ ................................ .... 3 9 操作步骤 ................................ ................................ ........... 4 样品采集 ................................ ................................ ....... 4 9.1 DNA提取 ................................ ................................ ........ 4 9.2 PCR扩增 ................................ ................................ ........ 4 9.3 PCR产物检测 ................................ ................................ .... 4 9.4 10 数据记录与统计 ................................ ................................ .... 5 数据记录 ................................ ................................ ...... 5 10.1 统计 ................................ ................................ .......... 5 10.2 11 结果判定 ................................ ................................ .......... 6 判定方法 ................................ ................................ ...... 6 11.1 结果表述 ................................ ................................ ...... 6 11.2 附录A（规范性） 引物序列信息 ................................ ......................... 7